Zhonglin Tang
Chinese Academy of Agricultural Sciences, China
Title: Systematic identification and characteristics of long non-coding RNAs in pigs
Biography
Biography: Zhonglin Tang
Abstract
The long non-coding RNAs (lncRNAs) are non-protein coding RNAs and involve in a variety of biological processes. Pig (Susscrofa) is an important farm animal and ideal biomedical model. In this study, we performed a genome-wide scan of lncRNAs in pig. Based on strand-specific RNA-Seq data, a computational method was used to identify 96,314 novel lncRNAs candidates in pig genome which included 39,528 large intergenic ncRNAs (lincRNAs), 180 nature antisense transcripts and 56,606 intronic ncRNAs. Novel lncRNAs show fewer exon number, shorter transcripts length, lower GC content, expression and conservation level, higher SNP density than protein-coding transcripts. To validate the result, we perform RT-PCR to detect selected lncRNAs. Expression analysis of 5 abundant lncRNAs was conducted by quantitative PCR (qPCR) in 9 tissues of Landrace pigs in 160 days post-coitus (dpc). We first performed identification and characterization of pig lncRNAs using strand specific RNA sequencing. Our study significantly expanded the number of pig lncRNAs in non-protein coding RNA database and provided important information for functional studies.
