Integrative Biology

Biography

Biography: Justyna Trynda

Abstract

MicroRNAs are short (~22 nucleotides) single strands of RNA that function as regulators of mRNA translation. They have integral roles in a broad array of biological processes. Many microRNAs are implicated in the anticancer action of calcitriol (active form of vitamin D). One of the most important is miR-125bknown as regulating vitamin D receptor (VDR) and vitamin D 24-hydroxylase- CYP24 expression.Moreover, miR-125bhave been reportedto be involved in a variety of cancers regulating cell cycle, differentiation and apoptosis by targeting a number of genes such as transcription factors, matrix-metalloproteases, members of Bcl-2 family, p53, NF-κB.The aim of our work was to study the role ofmiRNA-125bin anticancer activity of calcitriol in human leukemia and lymphoma cells. In our experiments we measured the expression of microRNA-125b after calcitriol treatment and correlated it withthe level of mRNA and protein e.g. vitamin D receptor, vitamin D 24-hydroxylase- CYP24, membrane associated rapid response steroid receptor ERp57,and others, which are responsible for differentiation of cells. We conclude that microRNA-125b may regulates crucial molecules engaged in differentiation process of human leukemia and lymphoma cells after exposure to calcitriol.