Integrative Biology

RNA-seq technology was performed by using a comparative transcriptome analysis of the MMTV-TGF-α female mice thymus tissues that were fed ad libitum (AL), chronic calorie restriction (CCR) (85% of AL fed mice) and intermittent calorie restriction (ICR) (3 weeks AL fed, 1 week 40% of AL fed mice) from 10 weeks of age to 17 weeks of age or 18 weeks of age. Th e results of RNA-seq analysis, a total of 6091 signifi cantly diff erentially expressed genes (DEGs) were identifi ed. 2821, 2825 and 445 signifi cantly DEGs were detected between AL-CCR, CCR-ICR and AL-ICR fed groups, respectively. Th ese DEGs were classifi ed according to cellular components, biological processes and molecular functions Gene Ontology (GO) main categories. 188 of 2821, 36 of 445, 176 of 2825 genes were identifi ed to be involved in immune system process (GO:0002376) biological processes GO categories. KEGG pathway and the gene co-expression network analysis between AL-CCR, CCRICR and AL-ICR fed groups immune-related DEGs were done using String database. For network analysis, nodes and edges

presented the interaction between immune-related DEGs.